公司简介：上海创赛科学仪器有限公司（Shanghai Canspec Scientific Instruments Co., Ltd.）（以下简称：上海创赛），是一家致力于实验室全套用品和设备（包括：生物、化学化工、实验耗材、仪器设备等）的集约化供应商。上海创赛作为综合性、专业性的集约化服务商，凭借优质的产品和先进的管理服务理念，为广大客户提供优质的产品和服务，赢得了客户和社会的信赖。上海创赛经过短短几年的发展，产品数量已经从10万种扩大至30万种有余，常规库存产品15万种，产品种类涉及有机化学、无机化学、高分子材料、化工中间体、催化剂、生命科学、检验检测、实验仪器、实验耗材、劳动保护等十大大产品线全面支持科研事业，为科研单位、高校、制药企业等提供全方位的一站式实验解决方案。目前，我司和很多家高等院校和科研单位建立了长期的合作关系如江南大学、温州医科大学、江苏大学、同济大学等。同时，上海创赛也建立了自己的自主知识产权商标PERFEMIKER（博飞美科）。

名称：Recombinant Human Tumor Necrosis Factor-alpha/TNFSF2

货号： C22-103-01-10ug

别名：rHuTNF-α/TNFSF2

Source: Escherichia coli. 

Molecular Weight: Approximately 17.5 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids. 

Quantity: 10μg/50μg/1000μg 

AA Sequence: MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL 

Purity: > 98 % by SDS-PAGE and HPLC analyses. 

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay 

using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107IU/mg in the presence of actinomycin D. 

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. 

Formulation: Lyophilized from a 0.2 ?m filtered concentrated solution in 20 mM PB, 10 mM Nacl, pH 7.0. 

Endotoxin: Less than 1.0 EU/?g of rHuTNF-α/TNFSF2 as determined by LAL method. 

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ?C. Further dilutions should be made in appropriate buffered solutions. 

Storage: This lyophilized preparation is stable at 2-8 ?C, but should be kept at -20 ?C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ?C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ?C to -70 ?C. Avoid repeated freeze/thaw cycles. 

Usage: This material is offered by Shanghai CANSPEC  Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE