产品信息

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form   tablet
packaging   pkg of 20 tablets (individually packed in foil blister packs)
mfr. no.   Roche
solubility   aqueous buffer: soluble
absorption   A/280 nm 0.08
shipped in   wet ice
price           3403.53

Features and Benefits

Use cOmplete Protease Inhibitor Tablets to protect your proteins from a wide range of proteases. In just minutes, uninhibited proteolytic activity can degrade the protein you have spent days isolating. Each of these convenient water-soluble tablets contains a blend of protease inhibitors that inhibits proteolytic activity from most cell types, including animals, plants, yeast, and bacteria. No weighing or measuring is necessary, ensuring consistent results.
? Convenience: Simply push the quick-dissolving tablet through the foil packaging of the EASYpack into your buffer.
? Complete protection: Instantly protect your proteins against a broad range of proteases.
? Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
? Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.

Application

cOmplete™, EDTA-free, efficiently inhibits serine and cysteine proteases in bacterial, yeast, plant, and animal cell extracts.[2][1][4][3] Metallo- and aspartic proteases are not inhibited.
cOmplete, EDTA-free Tablets, are used for the inhibition of proteolytic activity in large volumes (up to 50ml) in which EDTA may interfere with protein stability (e.g., metal-containing proteins) or subsequent assays. Since EDTA interferes with IMAC (Immobilized Metal Affinity Chromatography), cOmplete, EDTA-free is preferentially used in the isolation process of Poly-His-tagged fusion proteins.
If it is necessary to inhibit proteolytic activity in a smaller volume (up to 10ml), we recommend to use cOmplete, Mini, EDTA-free.

One cOmplete, EDTA-free tablet was added per 50 ml incubation solution. Proteolytic activity was determined with the Roche Universal Protease Substrate (casein, resorufin-labeled). When extractions or single-step isolations are necessary in the acid pH range, simply include pepstatin along with cOmplete, EDTA-free tablets to ensure aspartic (acid) protease inhibition. All experiments were performed at +15 to +25°C.

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